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jm109 e coli strains  (Addgene inc)


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    Addgene inc jm109 e coli strains
    Jm109 E Coli Strains, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jm109+e+coli+strains/JM109+(Bacterial+strain+%2349761)/10__1021_slash_acscatal__5c06530-233-6-41
    Average 93 stars, based on 15 article reviews
    jm109 e coli strains - by Bioz Stars, 2026-11
    93/100 stars

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    Cloning:

    Article Title: Valuable Compounds from Pollutants: Converting PET into Enantiopure Alanine
    Article Snippet: .. Competent NEB 10-beta (BioLabs Inc.) and JM109 E. coli strains were employed for gene cloning and plasmid propagation, E. coli BL21(DE3) cells were used for recombinant protein expression, and E. coli K-12 MG1655 RARE (reduced aromatic aldehyde reduction, bacterial strain #61440, Addgene, Watertown, USA) cells were used for whole-cell bioconversions. ..

    Plasmid Preparation:

    Article Title: Valuable Compounds from Pollutants: Converting PET into Enantiopure Alanine
    Article Snippet: .. Competent NEB 10-beta (BioLabs Inc.) and JM109 E. coli strains were employed for gene cloning and plasmid propagation, E. coli BL21(DE3) cells were used for recombinant protein expression, and E. coli K-12 MG1655 RARE (reduced aromatic aldehyde reduction, bacterial strain #61440, Addgene, Watertown, USA) cells were used for whole-cell bioconversions. ..

    Recombinant:

    Article Title: Valuable Compounds from Pollutants: Converting PET into Enantiopure Alanine
    Article Snippet: .. Competent NEB 10-beta (BioLabs Inc.) and JM109 E. coli strains were employed for gene cloning and plasmid propagation, E. coli BL21(DE3) cells were used for recombinant protein expression, and E. coli K-12 MG1655 RARE (reduced aromatic aldehyde reduction, bacterial strain #61440, Addgene, Watertown, USA) cells were used for whole-cell bioconversions. ..

    Expressing:

    Article Title: Valuable Compounds from Pollutants: Converting PET into Enantiopure Alanine
    Article Snippet: .. Competent NEB 10-beta (BioLabs Inc.) and JM109 E. coli strains were employed for gene cloning and plasmid propagation, E. coli BL21(DE3) cells were used for recombinant protein expression, and E. coli K-12 MG1655 RARE (reduced aromatic aldehyde reduction, bacterial strain #61440, Addgene, Watertown, USA) cells were used for whole-cell bioconversions. ..



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    Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.

    Journal: Applied and Environmental Microbiology

    Article Title: A recombineering-based platform for high-throughput genomic editing in Escherichia coli

    doi: 10.1128/aem.00193-25

    Figure Lengend Snippet: Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.

    Article Snippet: E. coli strains JM109 (ATCC 53323), MG1655 (ATCC 47076), JW128 , Nissle 1917 , and DH10B served as genetic materials in genomic editing experiments.

    Techniques: Sequencing